MB501, MB502, MB504 Midterm Current Papers

 

MB501, MB502, MB504 - Midterm Current Papers Fall 2023 -)





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Paper Review #1 - MB501

Long Qs

1: Explain peptide bond

2: Autosomal recessive Disorders

3: Explain Steps of PCR

Short Qs

1: prokaryotic cells

2: 3 RNA Viruses

3: How aneuploidy occurs in Meiosis

MCQs

How many traits Mendel study.??

Who invented PCR

Ribosomes collect at.??

 Cells were discovered by.??

Polyploid

Sex related chromosomes


Paper Review #2 - MB501

Mcqs from Zaman file mostly

Except

1. Bacterial DNA founds in?

Ma na necloide Kia hy.

2. Cellbolse kis sa banata hy?

3. Function of endoplasmic reticulum.

4. ABO blood group is an example of?

Small questions

1.Function of cell membrane

2. Difference between saturated and unsaturated fatty acids

3. Mutation define krna tha.

Long ques were

1.Functions of mitochondria

2. Define with examples

Incomplete dominance

Co dominance

Epitasis.

3. Glycosylation and proteins SA related koi word tha yad nai araha.


Paper Review #3 - MB501

💥Subjective

Shorts

1.write about cytoskeleton.

2.Functions of DNA.

long

1.Events of meiosis i and meiosis ii.

2. Discuss terms, peptide bond,

Primary structure and secondary structure of proteins.

💥 Mcqs

1.if one  gene prevents  the expression  of other gene.(epistasis)

2.example of  variable expressivity (huntington diseases).

3.Glucose converts into......( glycogen).

4.at equilibrium  stage  Go is equal to (zero).

5.charge on DNA is ??( neutral)

6.flavin is ......(co enzyme).

7.bacterioiophage is ......(virus).


Paper Review #4 - MB501

MCQS:

ions are formed by gain or loss of______

lipids are water_____

karyotype is defined as_____

in semiconservative model_____

in female heterozygous condition some traits do not show up because of____

okazaki fragments are joined by____

dynein and kinenson are example of____

at equilibrium delta G is ____

short questions:

1. what is active site of an enzyme?

2.what is the difference of character and trait?

3.what are the names of replication models of DNA?

long questions:

1. write models of replication in detail.

2. what is the role of cofactors, co enzymes and prosthetic groups in enzyme activity?

3. write difference of DNA and RNA.


Paper Review #5 - MB501

All mcqs from files

Short Questions

1.. what are exergonic reactions

2. What are multifactorial disorders

3. Define plasmids and it's function

Long Questions

1.. Functions of mitochondria

2.. Steps of PCR


Paper Review #6 - MB501

1.Functions of smooth endoplasmic reticulum.

2. Inhibitor enzymes k bary me pocha tha.

3.What is interphase. And it's importance. 

4. PCR k bary me pocha tha.

 5.conformational changes in enzyme and it's effect on enzyme activity.


Paper Review #7 - MB501

Short question:

1.Active site

2.metacentric chromosomes

Long

1. Transcription

2.  Conformational changes in enzymes

3.  Define cofactor coenzyme and prosthetics group?

Mcqs 1,2 file se the baqi pta Ni kha se the😒


Paper Review #8 - MB501

Mcqs

•           The inner membrane of mitochondria is called .....cristae.

•           Farmula of carbhydrates…

•           ENergy due to motion……kinetic

•           The tendency of an atom to attract electrons towards its….electronagtivity

•           Following has a major role in down Syndrome….Maternal age

•           Okazaki fragments are joined by the …..enzyme ligase.

•           Which is not cofactor…..NAD

•           Chromosomes are found in ….

•           At which Temperature denaturation occurs….95

•           Second law of mendel experiment ..

Short questions

1.         Which techniques is used in gel electrophoresis

2.         Composition of cytoskeleton

3.         Two laws of mendel experiment

Long questions

•           Note on technique of genetic engineering

•           Note on peptide bonds , primary protein structure and secondary protein structu

 

Paper Review #9 - MB501

Short Questions:

1-Functions of cell

2-Why Mendel use pea plant

3-Gel electro.....

Long:1-Epistasis,Codominance, Incomplete Dominance.

2-Semiconservative, Conservative and Disruptive Model of DNA replication

3-Peptide bond and primary Structure of protein


Paper Review #10 - MB501

Short question

💫1. Function of cell membrane?

💫2. Explain phases of mitosis?

💫3. What is DNA fingerprinting and its uses?

Long question

1. Explain cholesterol in detail?

2. Explain transcription?

3. Terms uniporters, antiporters and symporters? Also write difference?

McQs all from files that are already shared

Alhamdulillah easy


Paper Review #11 - MB501

Short questions:

Two main factors of protein

* Define karyotyy

* Gel electrophoresis

Long

Large site of ribosomes

Cholesterol and derivative

Main components of cell membrane


Paper Review #12 - MB501

Paper overall a bit  tricky McQs repeat huvy hain files say ..

Subjective was a bit hard

Short question

1)Phases of meiosis II

2)Amphiphatic Molecule

One was more but I didn’t remember

 Long question

1)Gloxyaltion and proteinolysis and their significance

2)Peptide bond? Primary structure,secondary structure ,tertiary structure and quaternary structure of proteins.

3Feedback machnsim by giving example of Kerb cycle

 

Paper Review #13 - MB501

MCQS

● *Kary Mullins* discovered PCR technology.

● Anything that occupy space and has mass is called *matter.*

● Function of golgi apparatus is *packaging & transport of proteins.*

 ● At equilibrium, Delta G becomes *zero.*

● Cycline D and Cycline E are produced during _____ phase.

1)G1  2)G2  3)G0  4)S

● Mendel was working with *pea plant.*

●ABO blood groups system is perfect example of *co-dominance.*

● Combination of DNA & proteins produce *ribosomes.*

● A nucleoside is *sugar & nitrogenous bases.*

● AGGAGG is called _______ sequence.

● How two non-polar groups can interact? *Hydrophibically.*

● Cellulose is stored form of glucose in *plants.*

● Ribosomes can be free or attached to *E.R*

● At *metaphase* chromosomes align along central axis of cell.

● Meiosis-II resembles *Mitosis*

Short Questions:

● Describe cell cycle.

● Name organelles found in cell.

● What factors influence migration of DNA in agarose gel electrophoresis?

Long Questions

● Explain concept of dominance observed by Mendel.

● Describe sites of larger subunit of ribosome.

● Explain term conformational changes in enzyme activity.


Paper Review #14 - MB501

*Short Question*

1. Glycosidic bond

2. Autosomal inheritance and sex linked inheritance.

3. Where transcription and traslation occur in prokaryotes.

 *Long Question*

1. Sites of larger subunit of ribosomes.

2. Difference between DNA and RNA.

3. Explain Cofactor, coenzymes, and Prosthetic group with Example.


Paper Review #1 - MB502

Mcqs from handouts and files 50 50%

Topoisomerases

Watson and crick model of DNA structure

AlkB in E.coli

Relationships of linked DNA with nucleosomes

Occurrence of bulges and loops in RNA

How many molecules used in UvrA, UvrB and UvRc in physiological condition


Paper Review #2 - MB502

1. Peptide bond

2. Name two scientists who first reported in 1933 that Drossophilla melanogater......... Aagy pta ni kiya likha hoa tha

3. Nuclear scafold

Long Question

1. How oxygen in cellular respiration (electron transport chain) promote DNA damage.

2. Define and explain tertiary strucure of protein.

3. How alflatoxin B1 lead to replication error.

Subjective Totally from handouts..

And MCQs were from Salman Ali file almost 90% .


Paper Review #3 - MB502

Questions

Linked DNA

Why cisplastin is cytotoxic and act as chemotherapeutic

Semi-conservative model

why oxygen formed by cellular respiration affects oxidative DNA

Discriminative amino acids

aik question yad ni a rha

MCQ's

DNA replication is semi-conservative was proved in __

Two mammalian 3’ exonuclease, TREX & TREX2 may convert the errors that introduced by___

Bond between nucleotide is___

Bond between pentose sugar and phosphate group is___

Which RNA bind with anticodon___

Amino acid attach to__ end of tRNA

tRNA having amino acid join with____ during protein synthesis_

The DNA connecting two nucleosomes is called linker DNA


Paper Review #4 - MB502

All mcqs from handouts

Subjective:

Q. Advancements of molecular biology in agriculture science

Q. DNA damage by aflatoxin B1.

Q. Water mediated deamination converts cytosine, guanine and adenine to ....

Q. Possible interpretation of Griffith's transformation experiments

Q. Replication error


Paper Review #1 - MB504

Short question

primers of nested pcr

Forensic science and fingerprinting

advantages of asymmetric pcr

Long questions

 Mini Primer PCR

Multiplex pcr in detail

Nested and Multiplex PCR difference

MCQ

1.----- determine specifity of PCR

2.Suicide pcr is used in

3.Allele specific pcr is used in identifying

4.-------- PCR is a variant of PCR which enables simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers.

5.which one is not type of PCR

6.----- pcr use primer whose 3' ends encompess the SNP.

7.------used to assemble two or more pieces of DNA into one piece.

8.tell structure of anealing from picture

9.Two pairs (instead of one pair) of PCR primers are used to amplify a fragment in -------- PCR.

10.RM stands for


Paper Review #2 - MB504

MCQS were from the ppts *Short Questions* 1. What are nested primers? What are there uses? 2. Give 2 uses of Asymmetrical PCR. 3. Differentiate between Type II and Type IIs restriction endonucleases. *Long Questions* 1. Differentiate between nested and RT PCR. 2. Differentiate between Asymmetrical, colony and In Situ PCR. 3. Write the applications of PCR in the field of microbiology.

 

Paper Review #3 - MB504

All mcqs from files

What do you have understanding about the term polymerase chain reaction?

Methodology of assembly pcr

Nomenclature of Smith and Nathan (1973)

Methodology of colony pcr

Type II and Type IIs of restriction modification (R-M) system

Reagents use in conventional pcr and reason of each reagent use

 


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